This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0055042
PrimePCR™ PreAmp for SYBR® Green Assay: PTCH1, Human
PrimePCR™ Template for SYBR® Green Assay: PTCH1, Human
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome esophageal squamous cell carcinoma trichoepitheliomas transitional cell carcinomas of the bladder as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.