PrimePCR™ SYBR® Green Assay: LRRK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0055264

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LRRK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LRRK2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048004
Assay Design:   exonic
Chromosome Location:   12:40671961-40677679question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000416796 ENST00000343742 ENST00000298910

Gene Information

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region a leucine-rich repeat (LRR) domain a kinase domain a DFG-like motif a RAS domain a GTPase domain a MLK-like domain and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq Jul 2008]

Gene Symbol:   LRRK2
Gene Name:   leucine-rich repeat kinase 2
Aliases:   AURA17, DARDARIN, PARK8, RIPK7, ROCO2
RefSeq:   NC_000012.11 NG_011709.1 NT_029419.12
Ensembl:   ENSG00000188906
Entrez:   120892
Chromosome Mapping:   12q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.850000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download