PrimePCR™ SYBR® Green Assay: FHIT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055290

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FHIT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FHIT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048030
Assay Design:   exonic
Chromosome Location:   3:59999774-59999875question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000575946 ENST00000476844 ENST00000492590 ENST00000468189 ENST00000488467 ENST00000341848 ENST00000329517 ENST00000339861 ENST00000455551 ENST00000420987 ENST00000545908 ENST00000216605 ENST00000555709 ENST00000322241 ENST00000228705 ENST00000249330 ENST00000313485 ENST00000273067 ENST00000452113 ENST00000435185 ENST00000457091 ENST00000336138 ENST00000571334 ENST00000570847 ENST00000573237 ENST00000571653 ENST00000293201

Gene Information

This gene a member of the histidine triad gene family encodes a diadenosine 5'5'''-P1P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3 where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact aberrant transcripts from this gene have been found in about half of all esophageal stomach and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Oct 2009]

Gene Symbol:   FHIT
Gene Name:   fragile histidine triad gene
Aliases:   AP3Aase, FRA3B
RefSeq:   NC_000003.11 NT_022517.18 NG_007551.1
Ensembl:   ENSG00000189283
Entrez:   2272
Chromosome Mapping:   3p14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.210000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download