PrimePCR™ SYBR® Green Assay: ADH7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055324


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PrimePCR™ PreAmp for SYBR® Green Assay: ADH7, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ADH7, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0048064
Assay Design:		exonic
Chromosome Location:		4:100348984-100349115question
Amplicon Length:		102
Splice Variants Targeted:		ENST00000437033 ENST00000482593 ENST00000476959 ENST00000474027 ENST00000209665

Gene Information

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver thus differing from the other known gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]

Gene Symbol:		ADH7
Gene Name:		alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
Aliases:		ADH4
RefSeq:		NC_000004.11 NT_016354.19
Ensembl:		ENSG00000196344
Entrez:		131
Chromosome Mapping:		4q23-q24

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	36.430000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.