PrimePCR™ SYBR® Green Assay: SCN8A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0055390


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PrimePCR™ PreAmp for SYBR® Green Assay: SCN8A, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SCN8A, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0048130
Assay Design:		exonic
Chromosome Location:		12:52099202-52099345question
Amplicon Length:		114
Splice Variants Targeted:		ENST00000550891 ENST00000354534 ENST00000355133 ENST00000551216 ENST00000545061

Gene Information

This gene encodes a member of the sodium channel alpha subunit gene family. The encoded protein forms the ion pore region of the voltage-gated sodium channel. This protein is essential for the rapid membrane depolarization that occurs during the formation of the action potential in excitable neurons. Mutations in this gene are associated with mental retardation pancerebellar atrophy and ataxia. Alternate splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:		SCN8A
Gene Name:		sodium channel, voltage gated, type VIII, alpha subunit
Aliases:		CERIII, MED, NaCh6, Nav1.6, PN4
RefSeq:		NC_000012.11 NT_029419.12 NG_021180.1
Ensembl:		ENSG00000196876
Entrez:		6334
Chromosome Mapping:		12q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.670000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.