PrimePCR™ SYBR® Green Assay: ZNF585A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055398

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF585A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF585A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048138
Assay Design:   exonic
Chromosome Location:   19:37646767-37646856question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000588873 ENST00000356958 ENST00000392157 ENST00000292841 ENST00000355533 ENST00000532828 ENST00000531805 ENST00000527838 ENST00000591492 ENST00000586320 ENST00000431078 ENST00000332149 ENST00000398585 ENST00000321871 ENST00000393130 ENST00000514894 ENST00000512662 ENST00000512137 ENST00000473312 ENST00000479901 ENST00000335367 ENST00000394229 ENST00000394230 ENST00000257880 ENST00000555728 ENST00000452168 ENST00000347027 ENST00000257879 ENST00000553804

Gene Information

Description Not Available

Gene Symbol:   ZNF585A
Gene Name:   zinc finger protein 585A
Aliases:   FLJ23765, FLJ25829, FLJ31827
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000196967
Entrez:   199704
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.620000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download