PrimePCR™ SYBR® Green Assay: CCL4L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055433

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CCL4L2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CCL4L2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048173
Assay Design:   exonic
Chromosome Location:   17:34640069-34640168question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000250151 ENST00000394495 ENST00000378350 ENST00000389068 ENST00000588929 ENST00000589079 ENST00000589336 ENST00000400702 ENST00000591167 ENST00000586598 ENST00000591637 ENST00000444414 ENST00000378352 ENST00000394465 ENST00000339270 ENST00000482104 ENST00000394463 ENST00000378342 ENST00000567608 ENST00000562236 ENST00000567807 ENST00000567858 ENST00000569856 ENST00000563132 ENST00000564234 ENST00000563802

Gene Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is similar to CCL4 which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals; most individuals have 1-5 copies in the diploid genome although rare individuals do not contain this gene at all. The human genome reference assembly contains two copies of this gene. This record represents the more telomeric gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CCL4L2
Gene Name:   chemokine (C-C motif) ligand 4-like 2
Aliases:   AT744.2, CCL4L, SCYA4L
RefSeq:   NC_000017.10 NG_004113.1 NT_010799.15
Ensembl:   ENSG00000197262
Entrez:   388372
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.993800
y-intercept 37.100000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download