PrimePCR™ SYBR® Green Assay: TRIM33, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055440

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRIM33, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRIM33, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048180
Assay Design:   exonic
Chromosome Location:   1:114968087-114968197question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000358465 ENST00000448034 ENST00000369543 ENST00000450349

Gene Information

The protein encoded by this gene is thought to be a transcriptional corepressor. However molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described however the full-length nature of one variant has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TRIM33
Gene Name:   tripartite motif containing 33
Aliases:   ECTO, FLJ32925, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA
RefSeq:   NC_000001.10 NG_023287.1 NT_032977.9
Ensembl:   ENSG00000197323
Entrez:   51592
Chromosome Mapping:   1p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.350000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download