PrimePCR™ SYBR® Green Assay: SLC22A6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0055505


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC22A6, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC22A6, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0048245
Assay Design:		exonic
Chromosome Location:		11:62747270-62747418question
Amplicon Length:		119
Splice Variants Targeted:		ENST00000360421 ENST00000377871 ENST00000458333 ENST00000421062

Gene Information

The protein encoded by this gene is involved in the sodium-dependent transport and excretion of organic anions some of which are potentially toxic. The encoded protein is an integral membrane protein and may be localized to the basolateral membrane. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		SLC22A6
Gene Name:		solute carrier family 22 (organic anion transporter), member 6
Aliases:		FLJ55736, HOAT1, MGC45260, OAT1, PAHT, ROAT1
RefSeq:		NC_000011.9 NT_167190.1
Ensembl:		ENSG00000197901
Entrez:		9356
Chromosome Mapping:		11q12.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.370000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.