PrimePCR™ SYBR® Green Assay: DHFR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055965

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DHFR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DHFR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048705
Assay Design:   exonic
Chromosome Location:   5:79924502-79924651question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000314636 ENST00000394221 ENST00000439211 ENST00000505337 ENST00000511032 ENST00000504396

Gene Information

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate a methyl group shuttle required for the de novo synthesis of purines thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5 multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq Jul 2008]

Gene Symbol:   DHFR
Gene Name:   dihydrofolate reductase
Aliases:   DHFRP1, DYR
RefSeq:   NC_000005.9 NG_016607.1 NG_023304.1 NT_006713.15
Ensembl:   ENSG00000228716
Entrez:   1719
Chromosome Mapping:   5q11.2-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.350000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download