PrimePCR™ SYBR® Green Assay: ZNF688, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055970

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF688, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF688, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048710
Assay Design:   exonic
Chromosome Location:   16:30581735-30582416question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000216083 ENST00000407418 ENST00000564535 ENST00000568089 ENST00000458477 ENST00000228307 ENST00000424649 ENST00000536957 ENST00000267257 ENST00000397506 ENST00000357503 ENST00000223459 ENST00000563276 ENST00000566632 ENST00000567855 ENST00000395219 ENST00000381605 ENST00000368088 ENST00000368073 ENST00000326837 ENST00000368074 ENST00000534366 ENST00000528919 ENST00000398656 ENST00000326134 ENST00000544576 ENST00000519602 ENST00000323997 ENST00000519584 ENST00000535453 ENST00000233607 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF688
Gene Name:   zinc finger protein 688
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000229809
Entrez:   146542
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.780000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download