PrimePCR™ SYBR® Green Assay: CLDND1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057503

List Price:    $183.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CLDND1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CLDND1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056398
Assay Design:   exonic
Chromosome Location:   3:98235634-98235908question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000437922 ENST00000514537 ENST00000510541 ENST00000508902 ENST00000512147 ENST00000502299 ENST00000513452 ENST00000511667 ENST00000513287 ENST00000511081 ENST00000510545 ENST00000513873 ENST00000394181 ENST00000394185 ENST00000503004 ENST00000394180 ENST00000341181

Gene Information

Description Not Available

Gene Symbol:   CLDND1
Gene Name:   claudin domain containing 1
Aliases:   C3orf4, GENX-3745, MGC111162, MGC3316, MGC9861
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000080822
Entrez:   56650
Chromosome Mapping:   3q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download