This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057507
PrimePCR™ PreAmp for SYBR® Green Assay: ACTN1, Human
PrimePCR™ Template for SYBR® Green Assay: ACTN1, Human
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions where it is involved in binding actin to the membrane. In contrast skeletal cardiac and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle cytoskeletal alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.