PrimePCR™ SYBR® Green Assay: ZNF286A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057556

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF286A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF286A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056451
Assay Design:   exonic
Chromosome Location:   17:15609715-15609806question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000301178 ENST00000359092 ENST00000593513 ENST00000391956 ENST00000221327 ENST00000592529 ENST00000586637 ENST00000591064 ENST00000583566 ENST00000472486 ENST00000395894 ENST00000580393 ENST00000585194 ENST00000464847 ENST00000395893 ENST00000481540 ENST00000593105 ENST00000421016 ENST00000585171 ENST00000570010 ENST00000464850 ENST00000332307 ENST00000393430 ENST00000567481 ENST00000591998 ENST00000308650 ENST00000588933 ENST00000588221 ENST00000592904 ENST00000590306 ENST00000592292 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF286A
Gene Name:   zinc finger protein 286A
Aliases:   KIAA1874, MGC149627, MGC156181, ZNF286
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000187607
Entrez:   57335
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.100000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download