PrimePCR™ SYBR® Green Assay: XPO1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0057703


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PrimePCR™ PreAmp for SYBR® Green Assay: XPO1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: XPO1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0056598
Assay Design:		exonic
Chromosome Location:		2:61726889-61726995question
Amplicon Length:		77
Splice Variants Targeted:		ENST00000401558 ENST00000404992 ENST00000406957 ENST00000451765

Gene Information

The protein encoded by this gene mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B MPAK and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq Jul 2008]

Gene Symbol:		XPO1
Gene Name:		exportin 1 (CRM1 homolog, yeast)
Aliases:		CRM1, DKFZp686B1823, emb
RefSeq:		NC_000002.11 NT_022184.15
Ensembl:		ENSG00000082898
Entrez:		7514
Chromosome Mapping:		2p16

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.720000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.