PrimePCR™ SYBR® Green Assay: EPB49, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057759

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EPB49, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EPB49, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056654
Assay Design:   exonic
Chromosome Location:   8:21938875-21938982question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000443491 ENST00000517600 ENST00000523782 ENST00000265800 ENST00000523266 ENST00000519907 ENST00000381470 ENST00000432128 ENST00000358242 ENST00000415253

Gene Information

Dematin or EPB49 is an actin-bundling protein originally identified in the erythroid membrane skeleton. Its actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase and is restored after dephosphorylation (Rana et al. 1993 [PubMed 8341682]).[supplied by OMIM Apr 2009]

Gene Symbol:   EPB49
Gene Name:   erythrocyte membrane protein band 4.9 (dematin)
Aliases:   DMT, FLJ78462, FLJ98848
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000158856
Entrez:   2039
Chromosome Mapping:   8p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.390000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download