PrimePCR™ SYBR® Green Assay: ABHD8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057786

List Price:    $188.00
Your Price:   Log In
 Bulk Discount Available - 25% off 5 or more Primer Based Assays
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ABHD8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

List Price:    $183.00
Your Price:   Log In
 Bulk Discount Available - 25% off 5 or more Primer Based Assays
Quantity:  
 

PrimePCR™ Template for SYBR® Green Assay: ABHD8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

List Price:    $188.00
Your Price:   Log In
 Bulk Discount Available - 25% off 5 or more Control Templates and Assays
Quantity:  
 

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056681
Assay Design:   exonic
Chromosome Location:   19:17403096-17403224question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000247706

Gene Information

This gene is upstream of and in a head-to-head orientation with the gene for the mitochondrial ribosomal protein L34. The predicted protein contains alpha/beta hydrolase fold and secretory lipase domains. [provided by RefSeq Jul 2008]

Gene Symbol:   ABHD8
Gene Name:   abhydrolase domain containing 8
Aliases:   FLJ11743, MGC14280, MGC2512
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000127220
Entrez:   79575
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.300000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download