PrimePCR™ SYBR® Green Assay: PNKP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057803

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PNKP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PNKP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056698
Assay Design:   exonic
Chromosome Location:   19:50368399-50368488question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000322344 ENST00000596014 ENST00000600910 ENST00000600573

Gene Information

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly seizures and developmental delay.[provided by RefSeq Sep 2010]

Gene Symbol:   PNKP
Gene Name:   polynucleotide kinase 3'-phosphatase
Aliases:   EIEE10, MCSZ, PNK
RefSeq:   NC_000019.9 NG_027717.1 NT_011109.16
Ensembl:   ENSG00000039650
Entrez:   11284
Chromosome Mapping:   19q13.3-q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.470000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download