PrimePCR™ SYBR® Green Assay: KIFC3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057852

List Price:    $188.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIFC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIFC3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056747
Assay Design:   exonic
Chromosome Location:   16:57798159-57799490question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000409429 ENST00000233668 ENST00000340004 ENST00000264012 ENST00000429102 ENST00000581171 ENST00000455895 ENST00000341071 ENST00000413080 ENST00000257013 ENST00000264501 ENST00000438707 ENST00000306802 ENST00000388738 ENST00000369902 ENST00000369899 ENST00000423559 ENST00000465878 ENST00000379655 ENST00000445690 ENST00000421376 ENST00000541240 ENST00000540079 ENST00000543930 ENST00000562903 ENST00000539578 ENST00000413420 ENST00000600009 ENST00000593285 ENST00000599270 ENST00000597902 ENST

Gene Information

KIFC3 belongs to the large superfamily of kinesins molecular motors that use the energy of ATP hydrolysis to translocate cargoes along microtubules. Members share extensive homology within a globular domain containing the microtubule- and ATP-binding sites and have a coiled-coil stalk domain that mediates oligomerization. Different kinesin family members participate in specific and diverse motile processes such as cell division organelle transport and nuclear movement (Hoang et al. 1998 [PubMed 9782090]).[supplied by OMIM Mar 2009]

Gene Symbol:   KIFC3
Gene Name:   kinesin family member C3
Aliases:   DKFZp686D23201, FLJ34694
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140859
Entrez:   3801
Chromosome Mapping:   16q13-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.660000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download