PrimePCR™ SYBR® Green Assay: STK25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057884

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: STK25, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: STK25, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056779
Assay Design:   exonic
Chromosome Location:   2:242441032-242447422question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000316586 ENST00000403346 ENST00000401869 ENST00000442307 ENST00000413760 ENST00000429279 ENST00000436402 ENST00000440109 ENST00000435225 ENST00000439101 ENST00000426941 ENST00000420551 ENST00000543554 ENST00000535007 ENST00000280333 ENST00000448039 ENST00000297954 ENST00000432730 ENST00000395477 ENST00000395475 ENST00000349097 ENST00000427277 ENST00000356055

Gene Information

Description Not Available

Gene Symbol:   STK25
Gene Name:   serine/threonine kinase 25
Aliases:   DKFZp686J1430, SOK1, YSK1
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000115694
Entrez:   10494
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.780000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download