PrimePCR™ SYBR® Green Assay: GAGE4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057900

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GAGE4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GAGE4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056795
Assay Design:   exonic
Chromosome Location:   X:49368280-49370604question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000407599 ENST00000442437 ENST00000381751 ENST00000402590 ENST00000404720 ENST00000415752 ENST00000381708 ENST00000381725 ENST00000420398 ENST00000405679 ENST00000381698 ENST00000440137 ENST00000445148 ENST00000381722 ENST00000362097 ENST00000381709 ENST00000376150 ENST00000244513 ENST00000305273 ENST00000381700 ENST00000594366 ENST00000597432 ENST00000593333 ENST00000600217 ENST00000600913 ENST00000593608 ENST00000601535 ENST00000601798 ENST00000596395 ENST00000593328 ENST00000595947 ENST

Gene Information

Description Not Available

Gene Symbol:   GAGE4
Gene Name:   G antigen 4
Aliases:   CT4.4
RefSeq:   NC_000023.9 NT_079573.3
Ensembl:   ENSG00000255738
Entrez:   2576
Chromosome Mapping:   Xp11.4-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.910000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download