PrimePCR™ SYBR® Green Assay: BHLHE23, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057951

List Price:    $188.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BHLHE23, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BHLHE23, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056846
Assay Design:   exonic
Chromosome Location:   20:61637624-61637807question
Amplicon Length:   154
Splice Variants Targeted:   ENST00000395353 ENST00000357402 ENST00000370346 ENST00000378330 ENST00000301030 ENST00000487664 ENST00000371757 ENST00000486577 ENST00000491806 ENST00000488444 ENST00000490355 ENST00000298480 ENST00000263604 ENST00000543963 ENST00000442466 ENST00000293892 ENST00000246117 ENST00000321870

Gene Information

Basic helix-loop-helix (bHLH) proteins such as BHLHB4 are transcriptional regulators.[supplied by OMIM Jul 2005]

Gene Symbol:   BHLHE23
Gene Name:   basic helix-loop-helix family, member e23
Aliases:   BETA4, BHLHB4, bA305P22.3
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000125533
Entrez:   128408
Chromosome Mapping:   20q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 38.840000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download