PrimePCR™ SYBR® Green Assay: CPLX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057952

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CPLX2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CPLX2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056847
Assay Design:   exonic
Chromosome Location:   5:175308340-175308449question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000359546

Gene Information

Proteins encoded by the complexin/synaphin gene family are cytosolic proteins that function in synaptic vesicle exocytosis. These proteins bind syntaxin part of the SNAP receptor. The protein product of this gene binds to the SNAP receptor complex and disrupts it allowing transmitter release. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CPLX2
Gene Name:   complexin 2
Aliases:   921-L, CPX-2, CPX2, Hfb1, MGC138492
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000145920
Entrez:   10814
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.260000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download