PrimePCR™ SYBR® Green Assay: MS4A14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058055

List Price:    $188.00
Your Price:   Log In
 Bulk Discount Available - 25% off 5 or more Primer Based Assays
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: MS4A14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

List Price:    $183.00
Your Price:   Log In
 Bulk Discount Available - 25% off 5 or more Primer Based Assays
Quantity:  
 

PrimePCR™ Template for SYBR® Green Assay: MS4A14, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

List Price:    $188.00
Your Price:   Log In
 Bulk Discount Available - 25% off 5 or more Control Templates and Assays
Quantity:  
 

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056950
Assay Design:   exonic
Chromosome Location:   11:60170414-60170510question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000531787 ENST00000300187 ENST00000395005 ENST00000526375 ENST00000531783 ENST00000534688 ENST00000395001

Gene Information

Description Not Available

Gene Symbol:   MS4A14
Gene Name:   membrane-spanning 4-domains, subfamily A, member 14
Aliases:   MGC104289, MGC49828, MS4A16, NYD-SP21
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000166928
Entrez:   84689
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download