PrimePCR™ SYBR® Green Assay: MTRF1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0058084


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PrimePCR™ PreAmp for SYBR® Green Assay: MTRF1L, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MTRF1L, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0056979
Assay Design:		exonic
Chromosome Location:		6:153309017-153309166question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000414771 ENST00000367233 ENST00000367231 ENST00000367230

Gene Information

Mitochondrial DNA encodes 13 proteins that play essential roles in the respiratory chain while all proteins involved in mitochondrial translation are encoded by nuclear genes that are imported from the cytoplasm. MTRF1L is a nuclear-encoded protein that functions as a releasing factor that recognizes termination codons and releases mitochondrial ribosomes from the synthesized protein (summary by Nozaki et al. 2008 [PubMed 18429816]).[supplied by OMIM Nov 2010]

Gene Symbol:		MTRF1L
Gene Name:		mitochondrial translational release factor 1-like
Aliases:		HMRF1L, MGC102748, MRF1L, mtRF1a
RefSeq:		NC_000006.11 NT_025741.15
Ensembl:		ENSG00000112031
Entrez:		54516
Chromosome Mapping:		6q25-q26

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.980000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.