PrimePCR™ SYBR® Green Assay: CASQ2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0058126


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PrimePCR™ PreAmp for SYBR® Green Assay: CASQ2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CASQ2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0057021
Assay Design:		exonic
Chromosome Location:		1:116243681-116243830question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000261448 ENST00000456138

Gene Information

The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2) a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq Jul 2008]

Gene Symbol:		CASQ2
Gene Name:		calsequestrin 2 (cardiac muscle)
Aliases:		FLJ26321, FLJ93514, PDIB2
RefSeq:		NC_000001.10 NG_008802.1 NG_016548.1 NT_032977.9
Ensembl:		ENSG00000118729
Entrez:		845
Chromosome Mapping:		1p13.3-p11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.500000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.