PrimePCR™ SYBR® Green Assay: RNF32, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058139

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RNF32, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RNF32, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057034
Assay Design:   exonic
Chromosome Location:   7:156447292-156450235question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000404282 ENST00000317955 ENST00000405335 ENST00000311822 ENST00000392741 ENST00000432459 ENST00000392743 ENST00000343665

Gene Information

The protein encoded by this gene contains two RING ring finger motifs. RING finger motifs are present in a variety of functionally distinct proteins and are known to be involved in protein-DNA or protein-protein interactions. This gene was found to be expressed during spermatogenesis most likely in spermatocytes and/or in spermatids. Several alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:   RNF32
Gene Name:   ring finger protein 32
Aliases:   FKSG33, HSD15
RefSeq:   NC_000007.13 NT_007741.14
Ensembl:   ENSG00000105982
Entrez:   140545
Chromosome Mapping:   7q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.270000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download