PrimePCR™ SYBR® Green Assay: SETD2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0058191


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PrimePCR™ PreAmp for SYBR® Green Assay: SETD2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SETD2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0057086
Assay Design:		exonic
Chromosome Location:		3:47164654-47164803question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000409792 ENST00000412450

Gene Information

Huntington's disease (HD) a neurodegenerative disorder characterized by loss of striatal neurons is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3 and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq Aug 2008]

Gene Symbol:		SETD2
Gene Name:		SET domain containing 2
Aliases:		FLJ16420, FLJ22472, FLJ23184, FLJ45883, FLJ46217, HBP231, HIF-1, HYPB, KIAA1732, KMT3A, SET2, p231HBP
RefSeq:		NC_000003.11 NT_022517.18
Ensembl:		ENSG00000181555
Entrez:		29072
Chromosome Mapping:		3p21.31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	36.040000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.