PrimePCR™ SYBR® Green Assay: SLC17A5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0058260


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC17A5, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC17A5, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0057155
Assay Design:		exonic
Chromosome Location:		6:74351450-74351589question
Amplicon Length:		110
Splice Variants Targeted:		ENST00000355773 ENST00000393019

Gene Information

This gene encodes a membrane transporter that exports free sialic acids that have been cleaved off of cell surface lipids and proteins from lysosomes. Mutations in this gene cause sialic acid storage diseases including infantile sialic acid storage disorder and and Salla disease an adult form. [provided by RefSeq Jul 2008]

Gene Symbol:		SLC17A5
Gene Name:		solute carrier family 17 (anion/sugar transporter), member 5
Aliases:		AST, FLJ22227, FLJ23268, ISSD, NSD, SD, SIALIN, SIASD, SLD
RefSeq:		NC_000006.11 NG_008272.1 NT_007299.13
Ensembl:		ENSG00000119899
Entrez:		26503
Chromosome Mapping:		6q14-q15

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	35.580000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.