PrimePCR™ SYBR® Green Assay: BCKDHB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058264

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BCKDHB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BCKDHB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057159
Assay Design:   exonic
Chromosome Location:   6:80878598-80878738question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000369760 ENST00000356489 ENST00000320393 ENST00000545529

Gene Information

Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1) lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit and mutations therein have been associated with maple syrup urine disease (MSUD) type 1B a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation and feeding problems. Alternative splicing at this locus results in transcript variants with different 3' non-coding regions but encoding the same isoform. [provided by RefSeq Jul 2008]

Gene Symbol:   BCKDHB
Gene Name:   branched chain keto acid dehydrogenase E1, beta polypeptide
Aliases:   E1B, FLJ17880, dJ279A18.1
RefSeq:   NC_000006.11 NG_009775.1 NT_007299.13
Ensembl:   ENSG00000083123
Entrez:   594
Chromosome Mapping:   6q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 40.970000
Efficiency 83

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download