PrimePCR™ SYBR® Green Assay: AUH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058271

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AUH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AUH, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057166
Assay Design:   exonic
Chromosome Location:   9:93979592-93983198question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000375731 ENST00000303617

Gene Information

The methylglutaconyl-CoA hydratase mitochondrial protein binds to the AU-rich element (ARE) a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase an enzyme involved in fatty acid degradation and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92% identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb and translated as a 40-kDa precursor protein which is subsequently processed to a 32-kDa mature form. [provided by RefSeq May 2010]

Gene Symbol:   AUH
Gene Name:   AU RNA binding protein/enoyl-CoA hydratase
Aliases:   Not Available
RefSeq:   NC_000009.11 NG_008017.1 NT_008470.19
Ensembl:   ENSG00000148090
Entrez:   549
Chromosome Mapping:   9q22.31

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