PrimePCR™ SYBR® Green Assay: BIK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058448

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BIK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BIK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057343
Assay Design:   exonic
Chromosome Location:   22:43520059-43523710question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000216115

Gene Information

The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins such as BID BAK BAD and BAX that is required for its pro-apoptotic activity and for interaction with anti-apoptotic members of the BCL2 family and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq Sep 2011]

Gene Symbol:   BIK
Gene Name:   BCL2-interacting killer (apoptosis-inducing)
Aliases:   BIP1, BP4, NBK
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100290
Entrez:   638
Chromosome Mapping:   22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.870000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download