PrimePCR™ SYBR® Green Assay: CEP290, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058456

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CEP290, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CEP290, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057351
Assay Design:   exonic
Chromosome Location:   12:88482924-88483071question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000547691 ENST00000552810 ENST00000309041 ENST00000397838

Gene Information

This gene encodes a protein with 13 putative coiled-coil domains a region with homology to SMC chromosome segregation ATPases six KID motifs three tropomyosin homology domains and an ATP/GTP binding site motif A. The protein is localized to the centrosome and cilia and has sites for N-glycosylation tyrosine sulfation phosphorylation N-myristoylation and amidation. Mutations in this gene have been associated with Joubert syndrome and nephronophthisis and the presence of antibodies against this protein is associated with several forms of cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   CEP290
Gene Name:   centrosomal protein 290kDa
Aliases:   3H11Ag, BBS14, CT87, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, POC3, SLSN6, rd16
RefSeq:   NG_021187.1 NC_000012.11 NG_008417.1 NT_029419.12
Ensembl:   ENSG00000198707
Entrez:   80184
Chromosome Mapping:   12q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.100000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download