PrimePCR™ SYBR® Green Assay: CCT7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best negative control; Same primer pair as used in probe assay qHsaCEP0058521


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PrimePCR™ PreAmp for SYBR® Green Assay: CCT7, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CCT7, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0057416
Assay Design:		exonic
Chromosome Location:		2:73478544-73479847question
Amplicon Length:		67
Splice Variants Targeted:		ENST00000258091 ENST00000398422 ENST00000540468 ENST00000539919 ENST00000537131 ENST00000538797

Gene Information

This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT) also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6. [provided by RefSeq Oct 2009]

Gene Symbol:		CCT7
Gene Name:		chaperonin containing TCP1, subunit 7 (eta)
Aliases:		CCTETA, CCTH, MGC110985, NIP7-1, TCP1ETA
RefSeq:		NC_000002.11 NT_022184.15
Ensembl:		ENSG00000135624
Entrez:		10574
Chromosome Mapping:		2p13.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.790000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.