PrimePCR™ Probe Assay: FCRLA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: FCRLA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FCRLA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041203
Assay Design:   exonic
Chromosome Location:   1:161683030-161683165question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000236938 ENST00000367957 ENST00000349527 ENST00000309691 ENST00000294796 ENST00000367953 ENST00000367950 ENST00000367959 ENST00000546024 ENST00000540521 ENST00000367949 ENST00000350710 ENST00000540926

Gene Information

This gene encodes a protein similar to receptors for the Fc fragment of gamma immunoglobulin (IgG). These receptors referred to as FCGRs mediate the destruction of IgG-coated antigens and of cells induced by antibodies. This encoded protein is selectively expressed in B cells and may be involved in their development. This protein may also be involved in the development of lymphomas. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   FCRLA
Gene Name:   Fc receptor-like A
Aliases:   FCRL, FCRL1, FCRLM1, FCRLX, FCRLb, FCRLc1, FCRLc2, FCRLd, FCRLe, FCRX, FREB, MGC4595
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000132185
Entrez:   84824
Chromosome Mapping:   1q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.890000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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