PrimePCR™ Probe Assay: TSEN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052910
Assay Design:   exonic
Chromosome Location:   3:12544808-12544951question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000446004 ENST00000454502 ENST00000402228 ENST00000284995 ENST00000415684 ENST00000314571 ENST00000383797 ENST00000444864

Gene Information

This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Feb 2009]

Gene Symbol:   TSEN2
Gene Name:   tRNA splicing endonuclease 2 homolog (S. cerevisiae)
Aliases:   MGC2776, MGC4440, PCH2B, SEN2, SEN2L
RefSeq:   NC_000003.11 NT_022517.18 NG_011521.1
Ensembl:   ENSG00000154743
Entrez:   80746
Chromosome Mapping:   3p25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.860000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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