PrimePCR™ SYBR® Green Assay: BCOR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0025947

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BCOR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BCOR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0005934
Assay Design:   Intron-spanning
Chromosome Location:   X:39914668-39916465question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000413905 ENST00000378463 ENST00000378455 ENST00000397354 ENST00000378444 ENST00000442018 ENST00000427012 ENST00000342274

Gene Information

The protein encoded by this gene was identified as an interacting corepressor of BCL6 a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6 but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein which suggests a possible link between the two classes of HDACs. Several transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome Y.[provided by RefSeq Jun 2010]

Gene Symbol:   BCOR
Gene Name:   BCL6 corepressor
Aliases:   ANOP2, FLJ20285, FLJ38041, KIAA1575, MAA2, MCOPS2, MGC131961, MGC71031
RefSeq:   NC_000023.10 NG_008880.1 NT_079573.4
Ensembl:   ENSG00000183337
Entrez:   54880
Chromosome Mapping:   Xp11.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download