PrimePCR™ SYBR® Green Assay: RLN3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: RLN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RLN3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006018
Assay Design:   Intron-spanning
Chromosome Location:   19:14139154-14141575question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000431365

Gene Information

Relaxins are known endocrine and autocrine/paracrine hormones belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes RLN1 RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. Relaxin is produced by the ovary and targets the mammalian reproductive system to ripen the cervix elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility regulating blood pressure controlling heart rate and releasing oxytocin and vasopressin. The protein encoded by this gene is a member of the relaxin family. The active form of the encoded protein consists of an A chain and a B chain but their cleavage sites are not definitely described yet. It may play a role in neuropeptide signaling processes. [provided by RefSeq Jul 2008]

Gene Symbol:   RLN3
Gene Name:   relaxin 3
Aliases:   H3, RXN3, ZINS4, insl7
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000171136
Entrez:   117579
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.940000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download