PrimePCR™ SYBR® Green Assay: SLC6A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026066

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC6A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC6A3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006207
Assay Design:   Intron-spanning
Chromosome Location:   5:1420696-1422046question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000270349 ENST00000513308 ENST00000453492

Gene Information

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat referred to as a variable number tandem repeat or VNTR which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy attention-deficit hyperactivity disorder dependence on alcohol and cocaine susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq Nov 2009]

Gene Symbol:   SLC6A3
Gene Name:   solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
Aliases:   DAT, DAT1
RefSeq:   NC_000005.9 NG_015885.1 NT_006576.16
Ensembl:   ENSG00000142319
Entrez:   6531
Chromosome Mapping:   5p15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.070000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download