This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: KDR, Human
PrimePCR™ Template for SYBR® Green Assay: KDR, Human
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor known as kinase insert domain receptor is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation survival migration tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors including Rab GTPase P2Y purine nucleotide receptor integrin alphaVbeta3 T-cell protein tyrosine phosphatase etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq May 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.