PrimePCR™ SYBR® Green Assay: MAP2K2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

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PrimePCR™ PreAmp for SYBR® Green Assay: MAP2K2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAP2K2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006314
Assay Design:   Intron-spanning
Chromosome Location:   19:4095417-4099262question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000262948 ENST00000394867

Gene Information

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome) a disease characterized by heart defects mental retardation and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene which is located on chromosome 7 has been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP2K2
Gene Name:   mitogen-activated protein kinase kinase 2
Aliases:   FLJ26075, MAPKK2, MEK2, MKK2, PRKMK2
RefSeq:   NC_000019.9 NG_007996.1 NT_011255.14
Ensembl:   ENSG00000126934
Entrez:   5605
Chromosome Mapping:   19p13.3

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