PrimePCR™ SYBR® Green Assay: SMC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: SMC2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SMC2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0006687
Assay Design:		Intron-spanning
Chromosome Location:		9:106862670-106864285question
Amplicon Length:		60
Splice Variants Targeted:		ENST00000440179 ENST00000374793 ENST00000374787 ENST00000286398 ENST00000303219 ENST00000536893

Gene Information

Members of the structural maintenance of chromosomes or SMC family (e.g. SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.[supplied by OMIM Nov 2010]

Gene Symbol:		SMC2
Gene Name:		structural maintenance of chromosomes 2
Aliases:		CAP-E, CAPE, FLJ10093, SMC2L1, hCAP-E
RefSeq:		NC_000009.11 NT_008470.19
Ensembl:		ENSG00000136824
Entrez:		10592
Chromosome Mapping:		9q31.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.995800
y-intercept	35.960000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.