PrimePCR™ SYBR® Green Assay: SENP6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: SENP6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SENP6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007054
Assay Design:   Intron-spanning
Chromosome Location:   6:76376572-76380321question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000370010 ENST00000327284 ENST00000447266 ENST00000424947 ENST00000370014 ENST00000538088

Gene Information

Ubiquitin-like molecules (UBLs) such as SUMO1 (UBL1; MIM 601912) are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin UBLs are synthesized as precursor proteins with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus the tail sequences of the UBL precursors need to be removed by UBL-specific proteases such as SENP6 prior to their conjugation to target proteins (Kim et al. 2000 [PubMed 10799485]). SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter 2008 [PubMed 18799455]).[supplied by OMIM Jun 2009]

Gene Symbol:   SENP6
Gene Name:   SUMO1/sentrin specific peptidase 6
Aliases:   FLJ11355, FLJ11887, KIAA0389, KIAA0797, SSP1, SUSP1
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000112701
Entrez:   26054
Chromosome Mapping:   6q13-q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.290000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download