PrimePCR™ SYBR® Green Assay: TBP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0036255

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TBP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007122
Assay Design:   Intron-spanning
Chromosome Location:   6:170873633-170876067question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000230354 ENST00000392092 ENST00000392091 ENST00000540980

Gene Information

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID) which binds to the core promoter to position the polymerase properly serves as the scaffold for assembly of the remainder of the transcription complex and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription serve as coactivators function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39 and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17 a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2010]

Gene Symbol:   TBP
Gene Name:   TATA box binding protein
Aliases:   GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID
RefSeq:   NC_000006.11 NT_025741.15 NG_008165.1
Ensembl:   ENSG00000112592
Entrez:   6908
Chromosome Mapping:   6q27

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