PrimePCR™ SYBR® Green Assay: VPS53, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007137
Assay Design:   Intron-spanning
Chromosome Location:   17:489510-505103question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000437048 ENST00000291074 ENST00000401468 ENST00000446250 ENST00000389040

Gene Information

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq Jul 2008]

Gene Symbol:   VPS53
Gene Name:   vacuolar protein sorting 53 homolog (S. cerevisiae)
Aliases:   FLJ10979, FLJ41112, FLJ61757, HCCS1, MGC39512, hVps53L, pp13624
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000141252
Entrez:   55275
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.600000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download