PrimePCR™ SYBR® Green Assay: WHSC1L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0026370


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PrimePCR™ PreAmp for SYBR® Green Assay: WHSC1L1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: WHSC1L1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0007170
Assay Design:		Intron-spanning
Chromosome Location:		8:38178612-38184319question
Amplicon Length:		121
Splice Variants Targeted:		ENST00000433384 ENST00000317025 ENST00000527502 ENST00000316985 ENST00000528627 ENST00000446459

Gene Information

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described. [provided by RefSeq Jul 2008]

Gene Symbol:		WHSC1L1
Gene Name:		Wolf-Hirschhorn syndrome candidate 1-like 1
Aliases:		DKFZp667H044, FLJ20353, MGC126766, MGC142029, NSD3, pp14328
RefSeq:		NC_000008.10 NT_167187.1 NG_023292.1
Ensembl:		ENSG00000147548
Entrez:		54904
Chromosome Mapping:		8p11.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.520000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.