PrimePCR™ SYBR® Green Assay: CSMD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026393

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CSMD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CSMD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007236
Assay Design:   Intron-spanning
Chromosome Location:   8:3008919-3015457question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000335551 ENST00000400186 ENST00000520002 ENST00000537824 ENST00000542608 ENST00000318252 ENST00000539096

Gene Information

Description Not Available

Gene Symbol:   CSMD1
Gene Name:   CUB and Sushi multiple domains 1
Aliases:   KIAA1890
RefSeq:   NC_000008.10 NT_023736.17
Ensembl:   ENSG00000183117
Entrez:   64478
Chromosome Mapping:   8p23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.991900
y-intercept 35.830000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download