PrimePCR™ SYBR® Green Assay: SETD1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026414

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SETD1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SETD1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007283
Assay Design:   Intron-spanning
Chromosome Location:   16:30970412-30972642question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000262519 ENST00000452917

Gene Information

SET1A is a component of a histone methyltransferase (HMT) complex that produces mono- di- and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).[supplied by OMIM Mar 2008]

Gene Symbol:   SETD1A
Gene Name:   SET domain containing 1A
Aliases:   KIAA0339, KMT2F, Set1, Set1A
RefSeq:   NC_000016.9 NG_012346.1 NT_010393.16
Ensembl:   ENSG00000099381
Entrez:   9739
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.220000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download