PrimePCR™ SYBR® Green Assay: MLL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026535

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MLL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MLL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007608
Assay Design:   Intron-spanning
Chromosome Location:   7:151849969-151851132question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000360104 ENST00000262189 ENST00000424877 ENST00000418061 ENST00000355193

Gene Information

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a nuclear protein with an AT hook DNA-binding domain a DHHC-type zinc finger six PHD-type zinc fingers a SET domain a post-SET domain and a RING-type zinc finger. This protein is a member of the ASC-2/NCOA6 complex (ASCOM) which possesses histone methylation activity and is involved in transcriptional coactivation. [provided by RefSeq Jul 2008]

Gene Symbol:   MLL3
Gene Name:   myeloid/lymphoid or mixed-lineage leukemia 3
Aliases:   DKFZp686C08112, FLJ12625, FLJ38309, HALR, KIAA1506, KMT2C, MGC119851, MGC119852, MGC119853
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000055609
Entrez:   58508
Chromosome Mapping:   7q36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 34.590000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download