PrimePCR™ SYBR® Green Assay: MRPL47, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: MRPL47, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MRPL47, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008090
Assay Design:   Intron-spanning
Chromosome Location:   3:179306679-179310484question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000476781 ENST00000259038 ENST00000392659

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a) in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   MRPL47
Gene Name:   mitochondrial ribosomal protein L47
Aliases:   MGC45403, NCM1
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000136522
Entrez:   57129
Chromosome Mapping:   3q26.33

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